Terbium Functionalized Schizochytrium-Derived Carbon Dots for Ratiometric Fluorescence Determination of the Anthrax Biomarker

Terbium Functionalized Schizochytrium-Derived Carbon Dots for Ratiometric Fluorescence Determination of the Anthrax Biomarker

Blog Article

Efficient and instant detection of biological threat-agent anthrax is highly desired in the fields of medical care and anti-terrorism.Herein, a new ratiometric fluorescence (FL) nanoprobe was elaborately tailored for the determination of 2,6-dipicolinic acid (DPA), a biomarker of anthrax spores, by grafting terbium ions (Tb3+) to the surface of carbon dots (CDs).CDs with blue FL were fabricated by Hoodie and sweatpants set a simple and green method using schizochytrium as precursor and served as an FL reference and a supporting substrate for coordination with Tb3+.On account of the absorbance energy transfer emission effect (AETE), green emission peaks of Tb3+ in CDs-Tb nanoprobe appeared at 545 nm upon the addition of DPA.Under optimal conditions, good linearity between the ratio FL intensity of F545/F445 and the concentrations of DPA was observed within the experimental concentration range of Hanovarian Bridle 0.

5−6 μM with the detection limit of 35.9 nM, which is superior to several literature studies and significantly lower than the infectious dosage of the Bacillus anthracis spores.Moreover, the CDs-Tb nanoprobe could sensitively detect DPA in the lake water sample.This work offers an efficient self-calibrating and background-free method for the determination of DPA.